Reporter

Part:BBa_K864120:Design

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-25)


pMB1 ori - RFP cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1463
    Illegal AgeI site found at 1575
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembled for use in low and medium copy backbones.


Source

The pMB1 origin has been PCR amplified from pSB1A3.


References